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fluorescent ros indicator l 012 sodium salt  (Tocris)


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    Tocris fluorescent ros indicator l 012 sodium salt
    Fluorescent Ros Indicator L 012 Sodium Salt, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent ros indicator l 012 sodium salt/product/Tocris
    Average 93 stars, based on 7 article reviews
    fluorescent ros indicator l 012 sodium salt - by Bioz Stars, 2026-04
    93/100 stars

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    Anti-IL36R treatment inhibits systemic inflammation in K14-IL17A ind mice. (A) Flow cytometry analysis of the bone marrow from Ctrl mice or K14-IL17A ind mice that either received IgG or anti-IL36R treatment (n = 2-3 animals per group). The relative percentage of positive cells is shown after pre-gating on viable cells. (B) Oxidative burst was measured in venous blood of control or K14-IL17A ind mice by analyzing the formation of reactive oxygen and nitrogen species. Blood cells were either restimulated with phorbol-12,13-dibutyrate (PDBu) or with Zymosan A. After addition of the <t>8-amino-5-chloro-7-phenylpyridol-(3,4-d)pyridazine-1,4-(2H,3H)-dione</t> sodium salt (L-012), the oxidative burst was detected by the created chemiluminescence using a SparkTM Multimode Microplate Reader. n = 4-5. C + D. Gene expression analysis of the spleen (C) and colon (D) from treated mice. Shown is the relative gene expression normalized to β-Actin (C) or Rpl37A (D) for n = 3-4 animals per group. Significance was calculated using a 2-tailed Student’s t-test: * p < 0.05, ** p < 0.01, *** p< 0.001. Shown is the mean ± SEM. (E) H&E staining of the colon at the endpoint. Scale: 100 µm.
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    Anti-IL36R treatment inhibits systemic inflammation in K14-IL17A ind mice. (A) Flow cytometry analysis of the bone marrow from Ctrl mice or K14-IL17A ind mice that either received IgG or anti-IL36R treatment (n = 2-3 animals per group). The relative percentage of positive cells is shown after pre-gating on viable cells. (B) Oxidative burst was measured in venous blood of control or K14-IL17A ind mice by analyzing the formation of reactive oxygen and nitrogen species. Blood cells were either restimulated with phorbol-12,13-dibutyrate (PDBu) or with Zymosan A. After addition of the 8-amino-5-chloro-7-phenylpyridol-(3,4-d)pyridazine-1,4-(2H,3H)-dione sodium salt (L-012), the oxidative burst was detected by the created chemiluminescence using a SparkTM Multimode Microplate Reader. n = 4-5. C + D. Gene expression analysis of the spleen (C) and colon (D) from treated mice. Shown is the relative gene expression normalized to β-Actin (C) or Rpl37A (D) for n = 3-4 animals per group. Significance was calculated using a 2-tailed Student’s t-test: * p < 0.05, ** p < 0.01, *** p< 0.001. Shown is the mean ± SEM. (E) H&E staining of the colon at the endpoint. Scale: 100 µm.

    Journal: Frontiers in Immunology

    Article Title: IL-17A-driven psoriasis is critically dependent on IL-36 signaling

    doi: 10.3389/fimmu.2023.1256133

    Figure Lengend Snippet: Anti-IL36R treatment inhibits systemic inflammation in K14-IL17A ind mice. (A) Flow cytometry analysis of the bone marrow from Ctrl mice or K14-IL17A ind mice that either received IgG or anti-IL36R treatment (n = 2-3 animals per group). The relative percentage of positive cells is shown after pre-gating on viable cells. (B) Oxidative burst was measured in venous blood of control or K14-IL17A ind mice by analyzing the formation of reactive oxygen and nitrogen species. Blood cells were either restimulated with phorbol-12,13-dibutyrate (PDBu) or with Zymosan A. After addition of the 8-amino-5-chloro-7-phenylpyridol-(3,4-d)pyridazine-1,4-(2H,3H)-dione sodium salt (L-012), the oxidative burst was detected by the created chemiluminescence using a SparkTM Multimode Microplate Reader. n = 4-5. C + D. Gene expression analysis of the spleen (C) and colon (D) from treated mice. Shown is the relative gene expression normalized to β-Actin (C) or Rpl37A (D) for n = 3-4 animals per group. Significance was calculated using a 2-tailed Student’s t-test: * p < 0.05, ** p < 0.01, *** p< 0.001. Shown is the mean ± SEM. (E) H&E staining of the colon at the endpoint. Scale: 100 µm.

    Article Snippet: After the addition of the chemiluminescence dye 8-amino-5-chloro-7-phenylpyridol-(3,4-d)pyridazine-1,4-(2H,3H)-dione sodium salt (L-012), the created chemiluminescence directly correlating with the underlying ROS formation was measured, using a Spark™ Multimode Microplate Reader (Tecan Trading AG, Männedorf, Switzerland).

    Techniques: Flow Cytometry, Expressing, Staining